In Silico tools was used for mutation analysis and prediction of secondary and tertiary structure of wild and mutant type genes.
The nucleotide sequences and translated proteins were subjected to BLAST for sequences similarity and homology, BLASTp result revealed a substitution of aspartic acid in TEM-1(gb: AFI61435.1) to Threonine at position 262.
This isolate was positive for TEM gene and negative for CTX-M and SHV genes. The ESBLs production was confirmed by phenotypic confirmatory test, while the ESBLs genes were detected by polymerase chain reaction (PCR). coli bacterium with high hydrolytic activity against cefotaxime. coli strains are of serious concern, since these organisms are the most common cause of different human infections. Correspondence to: Hisham N Altayb, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan. American Journal of Microbiological Research. Molecular Characterization and in Silico Analysis of a Novel Mutation in TEM-1 Beta-Lactamase Gene among Pathogenic E.
Mukhtar, Mohamed Ahmed Salih, Mohamed A M Siddig. 6, 217-223 DOI: 10.12691/ajmr-2-6-8 Copyright © 2014 Science and Education Publishing Cite this paper: Hisham N Altayb, Nagwa M El Amin, Maowia M. Mukhtar 1, Mohamed Ahmed Salih 3, Mohamed A M Siddig 4ġInstitute of Endemic Diseases, University of Khartoum, Khartoum, SudanĢDepartment of microbiology, Faculty of medicine, university of Khartoum, SudanģHead department of biotechnology, Biotechnology Park, Africa city of technology, SudanĤBotany department, Faculty of Science, University of Khartoum, Sudan American Journal of Microbiological Research. Hisham N Altayb 1,, Nagwa M El Amin 2, Maowia M.
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